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J Am Soc Nephrol 28(6):16861696 Tsalamandris S, Antonopoulos AS, Oikonomou E, Papamikroulis G-A, Vogiatzi G, Papaioannou S, Deftereos S, Tousoulis D (2019) The role of inflammation in diabetes: current concepts and future perspectives

Then, blood was sampled in the abdominal aorta and centrifuged for 15 min at 2000 g to obtain serum, which was subsequently retained and preserved at 80 C for subsequent analyses