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Next, the pH was adjusted to 6.0 and 4.0% (w/w) of papain was added at 50C for 60 min and stopping the enzyme at 100C for 5 min

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Anikster Y, Lucero C, Guo J, Huizing M, Shotelersuk V, Bernardini I, McDowell G, Iwata F, Kaiser-Kupfer MI, Jaffe R, Thoene J, Schneider JA, Gahl WA (2000) Ocular nonnephropathic cystinosis: clinical, biochemical, and molecular correlations