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Blood should be collected fasting, centrifuged promptly, and stored at -80C for optimal stability

The ALA-synthesizing capacities of ntrc, trxf1 and ntrc/trxf1 mutants were diminished by 36%, 15% and 80% compared to WT seedlings, respectively ( Figure 4D ), thus confirming that the absence of NTRC is responsible for impaired ALA synthesis and decreased Chl content in single and double mutants ( Figure 4 We then analyzed the stability and activity of GSAAT in order to verify the contribution of redox-dependent control to the regulation of ALA synthesis

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