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For best practices in a research setting, you should use one syringe for reconstitution and a fresh, sterile syringe (typically an insulin syringe) for drawing each precise dose for your experiment

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The p62-Keap1-NRF2 pathway plays an important role in preventing ferroptosis in HCC cells, and the RAS/Raf/MEK pathway is an important target for the treatment of HCC 32,40

Using a single insulin syringe for multiple draws within the same session (e.g., drawing once, recapping, re-drawing) increases contamination risk