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Immunoprecipitation Cells were lysed in ice-cold 0.5% NP-40 lysis buffer (50 mM TrisHCl, pH 8.0, 150 mM NaCl, 0.5% NP-40, 1 mM DTT) with protease inhibitors cocktail (Selleck, #B14001

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Prioritizing protein is essential to help preserve lean muscle mass while you lose body fat