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In an attempt to assess the impact of this fortification, Brito and colleagues (29) assessed the effect of a single intramuscular injection of 10 mg vitamin B-12 (plus 100 mg vitamin B-6 and 100 mg thiamin) in 51 Chilean asymptomatic vitamin B-12deficient older adults on changes in vitamin B-12 status determined by a combinedvitamin B-12 (c-B12) index, a calculated measure that combines the 4 blood biomarkers of vitamin B-12 status: total vitamin B-12, holotranscobalamin (holoTC), tHcy, and MMA (48)

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