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Selected 2D classes from this step were used as a reference for automated particle picking from entire datasets

Epididymitis typically results from retrograde pathogen ascent, most often bacterial [251, 252] although, non-infectious aetiologies such as vasculitis, autoimmune diseases, or medications (e.g., amiodarone) have been identified [253,254,255,256,257,258]

The supernatant was used for the estimation of malondialdehyde (MDA), protein thiol (SH) groups, glutathione total (GT), catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities