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The isolated cDNAs from the pool enriched in the fourth and fifth rounds were ligated into the pGEM-T-Easy vector (Promega), using DNA Ligation Kit Mighty Mix (Takara Bio), and cloned into DH5 competent cells

GHK-Cu treatment improved cognitive function in Alzheimers mice models, positioning it as a compound of interest in brain and central nervous system research

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The vitamin is naturally found in animal products such as meat, fish, dairy, and eggs, whilst fortified foods and supplements provide alternative sources for those with dietary restrictions or absorption difficulties