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GCL is specific for the glutamyl moiety and is regulated physiologically by: (a) non-allosteric feedback competitive inhibition by GSH (Ki=2.3mM), which involves binding of GSH to the glutamate and another site on the enzyme [8, 9] and (b) availability of its precursor, cysteine [1] .The apparent Km values of GCL for glutamate is 1.8 mM, and for cysteine and 0.10.3 mM [9]

The microplate was vortexed before measuring the samples at 750 nm using a microplate reader (Biotek Elx 800, USA)

Furthermore, BPC 157 can further alleviate the inflammatory response by inhibiting the NF-B signaling pathway and reducing the release of pro-inflammatory factors