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Visualization of the dynamics of gene expression in the living mouse
The Ni-NTA bead suspension was transferred to 5-ml disposable chromatography columns (29922, Thermo Fisher Scientific)
Once the Fmoc-protected amino acid attaches to the resin base, operators apply a chemical to remove the amino acid protecting group, allowing it to bond with the next activated amino acid
1.1 Choline uptake and utilization Choline is a vitamin-like essential quaternary ammonium salt present in the diet as free choline and phospholipid-bound forms
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