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Viability assay In 96-Well microplate, MCF-7 cells were incubated with various concentrations of PLGA nanoparticles (4500 g/mL) either empty (control) or encapsulating DDA-GSH-AuNCs (gold content of 0.79% w/w of PLGA) in Dulbeccos Modified Eagles Medium (DMEM) with 10% FBS for 24 hours