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After 4 h, glutathione levels are measured using the GSH-Glo Glutathione Assay (Promega, V6911) according to the manufacturers instructions

May 11, 2021;373:n1091 Available at:

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The newly identified 3G10 epitope involves amino acid residues from both N-Ag I and II sites, including VP1 GH-loop residues (Ser221, Asp226, Leu228), VP2 EF-loop residue (Thr168) from N-Ag II site, and VP1 BC-loop residues (Asn100, Lys101, Asp102) and EF-loop residue (Glu168) from N-Ag I site (Supplementary Tables 2 and 3), therefore suggesting that 3G10 targets a novel N-Ag site which is more complex than the classical N-Ag sites I to III