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The cell viability was measured using Trypan blue staining

Recommended guidance includes: Start with a low initial dose to support tolerability Gradual dose escalation over several weeks Maintain consistent weekly administration for best results Possible Side Effects Some individuals may experience mild to moderate side effects, particularly during dose escalation: Nausea Reduced appetite Gastrointestinal discomfort (bloating, constipation, diarrhea) Fatigue Headache Side effects are generally temporary and tend to subside as the body adjusts.

Pregnant or breastfeeding women are also advised against taking lipotropic injections, as the safety of these treatments during pregnancy and lactation has not been conclusively established

2000 Sep Nazzareno Ballatori, Suzanne M Krance, Sylvia Notenboom, Shujie Shi, Kim Tieu, Christine L Hammond