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Gradient elution starts from liquid A accounting for 100% of the mixture, during which the proportion of liquid B in the mixture gradually increases until it accounts for 100%, the flow rate is 10ml/min, and the detection wavelength is 215nm

* Correspondence: Kenjiro Hanaoka, [email protected] These authors have contributed equally to this work Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

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Its potential to improve metabolic health and facilitate sustained weight loss offers hope to many individuals struggling with obesity and related disorders