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One hour after refeeding, blood samples were collected via cheek bleeds or cardiac puncture, and plasma was isolated and snap-frozen for subsequent hormone analysis by ELISA
Antibodies (listed below) were prepared in phosphate buffered saline (PBS) with 0.1% Tween 20 and 3% bovine serum albumin (primary 1:1000
Generally speaking, the absorption efficiency after oral administration may be affected by the gastrointestinal environment, and different physiological states of the gastrointestinal tract may lead to differences in absorption rate and degree
Meta-analysis of randomized controlled trials on cognitive effects of Bacopa monnieri extract
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