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To immunoprecipitate DAT-GFP, the cell homogenates were incubated overnight at 4 C with a monoclonal anti-GFP antibody (G-6539, 1:100), followed by the addition of 50 l of a mixture of protein A and protein G Sepharose beads (2 h at 4 C in rotatory shaker)

However, individual responses vary based on genetic predispositions, lifestyle factors, and underlying metabolic conditions

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When present, side effects are typically mild and may include vivid dreams or altered sleep architecture as the brain undergoes increased neuroplastic activity, mild headaches during initial therapy (often resolving within days), temporary changes in appetite, or subtle mood variations during the adaptation period