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Cells convert NMN to NAD+ in one simple step through the enzyme NMN adenylyltransferase (NMNAT) [2]

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a , Lysates from HeLa cells that were either untreated or treated with UV (20 J/m2, 20 min recovery), 4-NQO (5 M, 1 h), MNNG (100 M, 1 h), MMS (10 mM, 30 min), Anisomycin (20 M, 1 h), Cycloheximide (100 g/mL, 1 h) or b , HU (4 mM, 4 h), Bleomycine (10 ug/mL, 1 h), IR (10 Gy, 30 min recovery), Calicheamicin (100 nM, 1 h) as shown in b were separated on Phos-tag SDS-PAGE and immunoblotted with the indicated antibodies

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