In addition, although comparison of absolute MRS neurochemical concentrations across different instruments and sites is highly challenging, the baseline absolute GSH levels that we observed (mean 0.109 IU (institutional units)
Blocking was followed by incubation with the primary antibodies, namely, anti-phospho-p38 MAPK (1:1000, Cell Signalling, 9211, Massachusetts, USA), anti-p38 MAPK (1:1000, Cell Signalling, 9212, Massachusetts, USA), anti-phospho-STAT6 (1:1000, Cell Signalling, 56554, Massachusetts, USA), anti-STAT6 (1:1000, Cell Signalling, 5397, Massachusetts, USA), anti-VEGFA (1:1000, Abcam, ab46154, Cambridge, UK), anti-IL8 (1:1000, Proteintech, 17038-1-AP, Chicago, USA), and anti--actin (1:5000, Proteintech, HRP-60008, Chicago, USA) at 4 C overnight
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