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The cell pellets were thawed and lysed in a buffer containing 20 mM HEPES, pH 7.5, 100 mM NaCl, 10% (v/v) glycerol, 10 mM MgCl 2 , 1 mM MnCl 2 and 100 M TCEP (Sigma-Aldrich) supplemented with EDTA-free protease inhibitor cocktail (TragetMol) by dounce homogenization

However, a recent study did not support this finding but rather attributed the increased risk of insulin resistance to the concomitant presence of metabolic dysfunctionassociated steatotic liver disease (MASLD) (81)

Adjacent compounds in peptide research include other synthetic peptides used for receptor binding assays, pharmacokinetic profiling, and formulation science