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Before use, make sure that the spray unit is perfectly inserted on the bottle

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Peptides and proteins were identified with a 1% false discovery rate (FDR) (Tang et al., LC-MS/MS Analysis in MRM Mode LC-MRM experiments were performed on an identical LC platform and column, and a QTRAP 5500 (Sciex, Concord, ON) hybrid quadrupole-linear ion trap system with a TurboIonSpray ion source in positive mode