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Since B12 is found primarily in animal products, including meat, fish, dairy, and eggs, a substantial reduction in food intake can reduce B12 consumption below optimal levels, particularly for patients who were already borderline deficient before starting treatment or whose diets lean toward plant-based foods

Use a dosage of 1 mcg/kg to 10 mcg/kg body weight for subcutaneous or intramuscular injection

Additionally, prepare single-use aliquots of reconstituted solution before storage to avoid repeated vial puncture and minimize contamination risk in longer experimental programs

This suggests that these vitamins may not be effective in reducing depression in this demographic