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One of the most common techniques in detecting oligomerization is coimmunoprecipitation, where pulling down one protomer also pulls down its dimer partner

Oxidative stress markers: By week 8-12, the glutathione peroxidase system is operating at a fully replenished capacity

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The western blot analysis for CGRP (rabbit anti-CGRP, BML-CA1134, Enzo Life Sciences, Farmingdale, NY, 1:2000) and TH (mouse anti-TH, sc-25269, Santa Cruz Biotechnology, Santa Cruz, CA, 1:1000) on protein extracts from renal pelvis were performed as described above with 12% SDS-PAGE gel to quantify the extent of ablation of afferent sensory nerves and determine if there was any damage to the efferent adrenergic innervation due to selective A-RDN in the pelvic wall of the kidney