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Lysates were cleared by centrifugation at 12,000 g for 15 min at 4 C and the supernatants were stored at 80 C until analysis

The main effects of this process are the breakdown of the cell nucleus and damage to the organelles.27 Apoptotic bodies then transform to lipofuscin under the action of heterogeneous lysosomes in the macrophages, which migrate into the mucosal lamina through the basement membrane

The nanoBRET ratio was calculated as the emission of the acceptor (618 nm) divided by the emission of the donor (460 nm)

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