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Chloroplasts Asc is also a cofactor for violaxanthin de-epoxidase where it participates within the production of xanthophylls which are directly involved in quenching of excessive excitatory energy on PS II (Davey et al., 2000)

The GST-tag was cleaved overnight by adding HRV 3C protease to the elution fraction, and the solution was dialysed against binding buffer overnight at 4 C

Weight fluctuates daily due to water retention, food volume, and hormonal cycles, so weekly averages provide better data than daily numbers

Thus, the results suggest that the use of Ferr-1 partially reversed the inhibitory effect of anisomycin on the viability of HuOCSCs cells, the decreased the extent of apoptosis, and necroptosis