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PVDF membranes were blocked with 5% skim milk at room temperature for 2 h and then incubated with primary antibodies at 4 C overnight, followed by incubation with secondary horseradish peroxidase-conjugated antibodies (1:1000, Sungene Biotech, China) for 1 h at room temperature

Reconstitution Instructions AOD-9604 is typically supplied as lyophilized (freeze-dried) powder in vials containing 5 mg of peptide

Zhou Q, Xu J, Yang S, Xue Y, Zhang T, Wang J, Xue C (2015a) The effect of various antioxidants on the degradation of o/w microemulsions containing esterified astaxanthins from Haematococcus pluvialis