The appearance of a pronucleus was used as the indicator of successful activation, suggesting that the oocyte had completed meiotic maturation and was capable of initiating embryonic development independently of fertilization
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For a 5 mg vial, adding 1-2 mL works because you will likely use the entire vial in one or two doses
The liquid should be clear and colorless (some compounded formulations may have a very slight tint, which is normal)