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Beads were washed with TSE-150 (0.1% SDS, 1% Triton, 2 mM EDTA, 20 mM Tris, pH 8.1, 150 mM NaCl), TSE-500 (as TSE-150 with 500 mM NaCl), LiCl detergent (0.25 M LiCl, 1% NP40, 1% Sodium Deoxycholate, 1 mM EDTA, 10 mM Tris, pH 8.1), and twice with Tris-EDTA (5:1 mM)

[DOI] [PubMed] [Google Scholar] 76.Marszaek M