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10.1016/j.redox.2025.103557 Redox Biol

However, it was not possible to obtain the initial results again, and this compound was also excluded from clinical trials ( in vitro , so its activity may be due to other mechanisms of action (Maples et al., 2001)

Griseri T, Arnold IC, Pearson C, Krausgruber T, Schiering C, Franchini F, et al

The tissue was finely minced using scissors and then digested in KRPH buffer (20 mM HEPES,5 mM KH2PO4,1 mM MgSO4.7H2),1mMCaCl2.2H2O,136 mM NaCl,4.7 mM KCL supplemented with 1 mg/ml collagenase A (Sigma-Aldrich) for 1 h at 37 C with gentle agitation