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Quantitative real-time PCR (qRT-PCR) analysis Total RNA was isolated from cortical samples by using RNAiso plus (Takara, Shiga, Japan) according to the manufacturers instructions

Clinical studies have associated carnosine supplementation with reductions in fatigue-related markers, including improved power maintenance and lower lactate accumulation within 68 weeks, supporting its role in short-duration, high-output activity models

Recent investigations from 2023 to 2025 highlight several key findings: microneedle-based delivery consistently reduced neuropathic pain [94], improved nerve function through precise local drug application [95], promoted regeneration when combined with stem cellderived products or electrically responsive systems [96], and decreased inflammation when loaded with anti-inflammatory agents [97]

Those guidelines do not define dosing strategies below the established initiation thresholds