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To further confirm Nrf2s involvement in the ER calcium regulation process via GPX8, in the same study, after silencing Nrf2 in HeLa cells, GPX8 mRNA and protein levels also decreased, resulting in elevated ER and cytosolic calcium levels at rest and increased histamine-induced ER calcium release (Granatiero et al., 2019)

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Further testing of antioxidative agents on these models would help to evaluate the safety and effectiveness of such treatment

Prior to study design that involved proteomic analysis, samples sizes were not considered prior to sampling