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Following post-hybridization stringency wash steps, sections were treated with 0.5% Triton X-100/0.5% H 2 O 2 in PBS for 15 min, rinsed in PBS, immersed in maleate buffer (0.1 M maleic acid, 0.15 M NaCl, pH 7.5

Rotate through them or mix and match to fit your routine

The antibody Fab7F38 appears to function by providing enhanced soluble surface area for crystal lattice packing, and indeed Fab7F38-bound GLP-1R can also assume an active conformation as shown by EM studies (see below), suggesting that Fab7F38 does not interfere with the conformational flexibility of the ECD during crystallization

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