In parallel, we cloned and purified 57 Cr GPDH 705 -FLAG variant with an additional 30 amino acids from the unstructured region at the N-terminus to eliminate the possibility that the GPP domain in the construct we used in our initial cellular studies was truncated due to potentially incorrectly predicted boundaries of the chloroplast targeting sequence (CTS) ( Figure 2a , Supplementary Figure S1 , Supplementary Figure S2b , Supplementary Figure S4 )
PubMed Central , pmc.ncbi.nlm.nih.gov/articles/PMC6380979/.
PMID: 35350465 Sikirica V, et al
This process involves both molecular and morphological changes in the astrocytes, including increased expression of GFAP, vimentin and nestin, uptake of excitotoxic glutamate, protection from oxidative stress by the production of GSH, neuroprotection by release of adenosine, degradation of amyloid-beta peptides, facilitation of blood-brain barrier, increased formation of gap junctions between astrocytes, formation of scars and, in some cases release of inflammatory cytokines, including tumor necrosis factor- (TNF-), and production of ROS [3,35,38-40]