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These results suggest that Sema can counteract the TAC-induced activation of the PI3K/AKT/Creb5/NR4a1 signaling pathway and reduce NR4a1 nuclear-mitochondrial translocation to ameliorate TAC-induced cardiac remodeling

To remove any remaining blood, blood vessels were rinsed with cold PBS three times (by using a syringe) and were left for additional 5 minutes in the 50 mL beaker filled with the cold PBS solution under dark conditions

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For the 8mg/mL concentration, a 1 mL syringe is necessary for any dose above 2.5mg