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Cellular mechanisms for heavy metal detoxification and tolerance

Immunofluorescence (IF) for PML bodies 21NT cells (treated with 1ug/ul epitalon and untreated) were plated onto glass microscope slides (Thermo Scientific), then fixed in 2% formaldehyde (Fisher Scientific) for 15 min and cold methanol for 10 min

When you store it outside the recommended temperature range, those bonds begin to break down through processes called oxidation, hydrolysis, and aggregation

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