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On day 2, slices were washed 35 min in PBS 0.01 M at RT 4 mL/well and incubated in donkey anti-guinea pig-AF647 (1:500, Jackson ImmunoResearch) diluted in [PBS 0.01 M + 0.3% Triton]

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Brain Endothelial Cells Regulate Glucagon-Like Peptide 1 Entry Into the Brain via a Receptor-Mediated Process

When the synthesis of GSH synthesis is blocked, the activity of GPX4 is decreased, as is the cellular antioxidant capacity, which leads to the accumulation of ROS and promotes ferroptosis (39)