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The specific time of day does not significantly affect efficacy, but consistency helps establish a routine

The catheters were exteriorized through the dorsal skin and flushed with heparinized saline before and after blood sample collection and filled with heparin-glycerol locking solution (6 mL glycerol, 3 mL saline, 0.5 mL gentamycin (l OOmg/mL), 0.5 mL heparin (10,000 u/mL)) when not sampled for more than 8 hours

Adults require approximately 1.5 micrograms daily, but those at risk (vegans, older adults, or those with absorption problems) should take regular supplements or consume fortified foods to maintain optimal levels

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