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A microplate reader (Synergy Mx, Biotek Instruments Inc., Winooski, VT, USA) were used for determination of all activities and scaled down for semi-high throughput to obtain linear time and protein concentration dependence

Nasal sprays contain active pharmaceutical ingredients, for either systemic or local effects, in the form of a solution or suspension with excipients (e.g., preservatives, buffering agents, viscosity modifiers, emulsifiers), filled in non-pressurized dispensers that deliver a spray containing a metered dose of the drug

This green synthesis approach reduces energy consumption, avoids toxic byproducts, and enhances biocompatibility and antimicrobial efficacy

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