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In twin pregnancies, this situation happens more frequently due to: Faster hCG multiplication Higher hormone concentration earlier in gestation Limited detection range of standard urine kits As a result, the test fails to register hCG accurately, and the outcome is a false negative

Phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway is essential in the promotion of cellular growth, proliferation, and longevity