After five minutes, initiate the reaction by adding 20 L of the Cumene Hydroperoxide Solution into each well and mix thoroughly (it is recommended to use a multi-channel pipette)
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Proximity ligation assay (PLA) for Cntfr and Syt1 Labelling of Cntfr was performed as described above also including mouse anti-Syt1 luminal antibodies (1:150 dilution, Synaptic Systems, cat
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