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Following Matrigel polymerization for 10 min at 37 C, advanced DMEM/F12 was supplemented with penicillin/streptomycin, 2 mM GlutaMAX, 10 mM HEPES, 1 B27, 1 N2 (Life Technologies), 1mM N-acetylcysteine (Sigma), and 10 nM gastrin I (Biogems)

It is important to note that B12 levels should ideally be measured before commencing replacement therapy, as supplementation rapidly normalises biochemical markers, potentially obscuring the diagnosis

Lifting the midface using a hyaluronic acid filler with lidocaine: A randomized multi-center study in a Chinese population, Journal of Cosmetic Dermatology, 21(12), pp

Notably, 3D culture conditions are recognized for its superior ability to predict in vivo efficacy to anticancer drugs than 2D culture in liquid medium [4, 5]