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Lancet Diabetes Endocrinol 7(8):596605

Yet, the interaction mechanism between glutamine (Gln) and glucose remains ambiguous

Male mice lacking the entire Mc4r coding region were fertilized with oocytes from female C57BL/6J mice by in vitro fertilization (IVF) to generate heterozygous offspring of both sexes, which were subsequently intercrossed to obtain homozygous knockout mice