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Glucose and insulin levels in the tail vein blood were determined by GlucoCard DIA meter and insulin ELISA kit (Morinaga)

Nasal sprays formulated outside the isotonic range (270330 mOsm/kg) can cause irritation and impair the nasal mucociliary clearance apparatus the cilia-driven transport system that moves mucus, particles, and pathogens from the nasal cavity toward the nasopharynx

These experiments were performed using a Superdex 200 Increase SD200 column (Cytiva) and 20 mM HEPES pH 8, 200 mM NaCl, 1 mM DTT