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doi: 10.1016/S0140-6736(19)31150-X 15 GersteinH

The following are known triggers of mast cell degranulation (in addition to IgG activation via pathogen or allergen):[ref] Acetylcholine Complement fragments (C3, C4, C5) Various drugs, environmental toxins (MRGPRX2 receptor, details below) Peptides (including endorphin, leptin, PTH, and more) Physical conditions (cold, heat, pressure, stress, and vibration)

In fact, fat-free mass lost accounts for 25-40% of the total pounds lost, while age-related declines in fat-free mass are only 8% per decade