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Other side effects include the accumulation of toxins which can damage the liver, anorexia or loss of appetite, vomiting, azotemia -- elevation of blood urea nitrogen (BUN) -- and the development of a dry cough due to the accumulation of bradykinin
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In the jump dilution assay, after incubation of enzyme, NADPH, and inhibitor, the reactions are diluted to well below the IC 50 for the inhibitor allowing its release from the enzyme and activity is determined
While phosphorylation at Ser616 enhances fission activity, phosphorylation at Ser637 exerts an inhibitory effect under physiological conditions (Longo et al., 2021)
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