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Editor information Editors and Affiliations Rights and permissions Copyright information 2015 Springer Science+Business Media New York About this protocol Cite this protocol Vikis, H.G., Guan, KL

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After 10 days, the number of wells containing spheres was determined for each condition and the software ELDA (Extreme limiting dilution analysis) was used to calculate the long-term cancer stem cell initiating frequency and significance [4]