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Branched-chain amino acids serve as the major nitrogen donor for glutamate synthesis, playing a buffering role in post-traumatic brain injury glutamate elevation, with leucine contributing nearly 50% of nitrogen in the brain (106)

After quality control based on Qubit 4.0 and Tapestation analysis, libraries were pooled together in an equimolar manner and sequenced on two high-output runs in a single-end mode (186 bp) using a NextSeq500 Illumina sequencer