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Although the magnitude of hydraulic resistance experienced by migrating breast cancer cells in vivo is currently unknown, mathematical modeling indicates that depending on the interstitial fluid viscosity and matrix permeability, the hydraulic resistance within 3D matrices could potentially match or even exceed that observed in microchannels [50, 203]

For example, granulosa cells transport glycolytic metabolites, such as pyruvate, to oocytes, where they are further metabolized into ATP within mitochondria [47, 48]

Glucogenic amino acids are those that give rise to a net production of pyruvate or TCA cycle intermediates, such as 2-oxoglutarate (-ketoglutarate) or oxaloacetate, all of which are precursors to glucose via gluconeogenesis

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