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The assay was carried out in the presence of mouse retinal lysate containing an enzyme buffer mixture (50 mM Tris-HCl [pH 7.4], 100 mM KCl, 5 mM MgCl 2 , 1 mM ADP, 0.5 mM PEP, and 0.2 mM NADH [reduced form of NAD + ]) and 8 U of LDH with a reaction volume of 1.0 ml

Berberine also lowers your blood pressure, so combining the two can dangerously lower your blood pressure levels

Several studies have proven the anti-inflammatory potential of NTZ as it downregulates proinflammatory cytokines in LPS-induced systemic inflammation (Vanessa Legry et al., 2022), ovariectomized mice (Li et al., 2021), and type-2 diabetic patients (Castillo-Salazar et al., 2021) which supports our findings