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IL-33 can be released in the extracellular space after cell damage (necrotic cell death) or mechanical injury [10, 11], and binds to its receptor ST2

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549 The reactive astrocytes can promote excitotoxic injury of motor neurons by producing nitric oxide and peroxynitrite, which cause mitochondrial damage and apoptosis in cultured neurons, decreasing glutamate transport, releasing pro-apoptotic mediators selectively toxic to motor neurons

These modifications significantly reduce IGF-1 LR3's binding to IGF binding proteins while maintaining high affinity for the IGF-1 receptor, making it a powerful research tool for studying cell proliferation, differentiation, and survival signalling pathways