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Figure 2 The clinical hallmark and primary cause of SCD hospitalisations are vaso-occlusive crises (VOCs) (Rees et al., 2010

Therefore, we believe the one-time burden estimate to implement the new requirements finalized in this final rule with comment period to be 88,992 hours for all hospitals (12 hours 7,416 hospitals) at a cost of $10,840,708.80 (7,416 hospitals [($87.52 8 hours) + ($128.00 2 hours) + ($252.82 2 hours])

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Furthermore, the PCR amplicon sizes for the 5 check and 3 check of GDH3 gene disruption, 600 and 790 bp, matched the predicted values of 607 and 751 bp, respectively